Western blotting analysis using RNF139 antibody (Cat#5268). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RNF139 antibody (Cat#5268, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
RNF139; Ring Finger Protein 139; TRC8; HRCA1; RCA1; Translocation In Renal Carcinoma On Chromosome 8 Protein; Translocation In Renal Carcinoma, Chromosome 8; RING-Type E3 Ubiquitin Transferase RNF139; E3 Ubiquitin-Protein Ligase RNF139; Patched Related Protein Translocated In Renal Cancer; Multiple Membrane Spanning Receptor TRC8; RING Finger Protein 139; EC 2.3.2.27
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:400-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using RNF139 antibody (Cat#5268). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RNF139 antibody (Cat#5268, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using RNF139 antibody (Cat#5268). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RNF139 antibody (Cat#5268, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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