TRIM21 Mouse mAb - Product - Hefei GenuIN Biotech Co., Ltd.

TRIM21 Mouse mAb

#5282

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Western blotting analysis using TRIM21 antibody (Cat#5282). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TRIM21 antibody (Cat#5282, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Immunocytochemical staining of HT-1080 cells with TRIM21 antibody (Cat#5282, 1:1,000). Nuclei were stained blue with DAPI; TRIM21 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.

Flow cytometric analysis of TRIM21 expression in HT-1080 cells using TRIM21 antibody (Cat#5282, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, TRIM21.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$69.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

TRIM21
Catalog：

5282
Application：

WB, FCM, ICC
Species Reactivity：

H

Information

Data

Information

Product Name	TRIM21 Mouse mAb
Aliases	TRIM21; Tripartite Motif Containing 21; RNF81; RO52; E3 Ubiquitin-Protein Ligase TRIM21; SSA1; Sjogren Syndrome Antigen A1 (52kDa, Ribonucleoprotein Autoantigen SS-A/Ro); 52 KDa Ribonucleoprotein Autoantigen Ro/SS-A; Tripartite Motif-Containing Protein 21; Sjoegren Syndrome Type A Antigen; RING Finger Protein 81; 52 KDa Ro Protein; Ro/SSA 52kDa; Ro(SS-A); SS-A; RING-Type E3 Ubiquitin Transferase TRIM21; Tripartite Motif-Containing 21; Sicca Syndrome Antigen A; EC 2.3.2.27; Ro/SSA; SSA
Background	Gene Name: TRIM21 NCBI Gene Entry: 6737 UniProt Entry: P19474
Application Information	Molecular Weight: Predicted, 54 kDa; observed, 50 kDa Clonality: Mouse monoclonal antibody Clone ID: 25GB735 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen	Recombinant protein of human TRIM21
Isotype	Mouse IgG1
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:400-1:2,000 Flow Cytometry (FCM): 1:200-1:2,000 Immunocytochemistry (ICC): 1:100-1:1,000
Note	This product is for research use only.

Data

Western blotting analysis using TRIM21 antibody (Cat#5282). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TRIM21 antibody (Cat#5282, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with TRIM21 antibody (Cat#5282, 1:1,000). Nuclei were stained blue with DAPI; TRIM21 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of TRIM21 expression in HT-1080 cells using TRIM21 antibody (Cat#5282, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, TRIM21.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$69.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

TRIM21
Catalog：

5282
Application：

WB, FCM, ICC
Species Reactivity：

H

Datasheet

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