Western blotting analysis using ABCF2 antibody (Cat#5757). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ABCF2 antibody (Cat#5757, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of ABCF2 expression in C2C12 cells using ABCF2 antibody (Cat#5757, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ABCF2.
ABCF2; ATP Binding Cassette Subfamily F Member 2; EST133090; HUSSY-18; ABC28; ATP-Binding Cassette, Sub-Family F (GCN20), Member 2; ATP-Binding Cassette Sub-Family F Member 2; Iron-Inhibited ABC Transporter 2; M-ABC1; ABC-Type Transport Protein; EC 3.6.3.17; EC 3.6.3; HUSSY18
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human ABCF2
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using ABCF2 antibody (Cat#5757). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ABCF2 antibody (Cat#5757, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of ABCF2 expression in C2C12 cells using ABCF2 antibody (Cat#5757, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ABCF2.
Western blotting analysis using ABCF2 antibody (Cat#5757). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ABCF2 antibody (Cat#5757, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of ABCF2 expression in C2C12 cells using ABCF2 antibody (Cat#5757, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ABCF2.
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