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Home>Product>Primary Antibody

RECQL Mouse Oligoclonal Antibody

#5296

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Western blotting analysis using RECQL antibody (Cat#5296). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RECQL antibody (Cat#5296, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with RECQL antibody (Cat#5296, 1:1,000). Nuclei were stained blue with DAPI; RECQL was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of RECQL expression in HepG2 cells using RECQL antibody (Cat#5296, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, RECQL.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RECQL
  • Catalog：
    5296
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name RECQL Mouse Oligoclonal Antibody
Aliases RECQL; RecQ Like Helicase; RecQ1; RecQL1; RecQ Protein-Like (DNA Helicase Q1-Like); DNA Helicase, RecQ-Like Type 1; ATP-Dependent DNA Helicase Q1; DNA-Dependent ATPase Q1; DNA 3'-5' Helicase Q1; RecQ Protein-Like 1; RecQ Helicase-Like; DNA Helicase Q1-Like; EC 5.6.2.4; EC 3.6.1; RECQL1; RECON; RECQ1
Background

Gene Name: RECQL

NCBI Gene Entry: 5965

UniProt Entry: P46063

Application Information

Molecular Weight: Predicted, 73 kDa; observed, 70 kDa

Clonality: Mouse oligoclonal antibody

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human RECQL
Isotype Mouse IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using RECQL antibody (Cat#5296). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RECQL antibody (Cat#5296, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with RECQL antibody (Cat#5296, 1:1,000). Nuclei were stained blue with DAPI; RECQL was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of RECQL expression in HepG2 cells using RECQL antibody (Cat#5296, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, RECQL.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RECQL
  • Catalog：
    5296
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using RECQL antibody (Cat#5296). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RECQL antibody (Cat#5296, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with RECQL antibody (Cat#5296, 1:1,000). Nuclei were stained blue with DAPI; RECQL was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of RECQL expression in HepG2 cells using RECQL antibody (Cat#5296, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, RECQL.
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