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KD-Validated Lactate Dehydrogenase C Recombinant Rabbit mAb

#65579

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using lactate dehydrogenase C antibody (Cat#65579). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with lactate dehydrogenase C antibody (Cat#65579, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using lactate dehydrogenase C antibody (Cat#65579). Lactate dehydrogenase C expression in wild-type (WT) and lactate dehydrogenase C (LDHC) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with lactate dehydrogenase C antibody (Cat#65579, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification:
Unit price:
$89.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    LDHC
  • Catalog:
    65579
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name KD-Validated Lactate Dehydrogenase C Recombinant Rabbit mAb
Aliases LDHC; Lactate Dehydrogenase C; CT32; Cancer/Testis Antigen 32; L-Lactate Dehydrogenase C Chain; LDH Testis Subunit; EC 1.1.1.27; LDH-C; LDH-X; LDH3; LDHX; Testis Secretory Sperm-Binding Protein Li 234P; Lactate Dehydrogenase C4; EC 1.1.1
Background

Gene Name: LDHC

NCBI Gene Entry: 3948

UniProt Entry: P07864

Application Information

Molecular Weight: Predicted, 36 kDa; observed, 31 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB4355

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human Lactate Dehydrogenase C
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using lactate dehydrogenase C antibody (Cat#65579). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with lactate dehydrogenase C antibody (Cat#65579, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using lactate dehydrogenase C antibody (Cat#65579). Lactate dehydrogenase C expression in wild-type (WT) and lactate dehydrogenase C (LDHC) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with lactate dehydrogenase C antibody (Cat#65579, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using lactate dehydrogenase C antibody (Cat#65579). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with lactate dehydrogenase C antibody (Cat#65579, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using lactate dehydrogenase C antibody (Cat#65579). Lactate dehydrogenase C expression in wild-type (WT) and lactate dehydrogenase C (LDHC) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with lactate dehydrogenase C antibody (Cat#65579, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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