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Home>Product>Primary Antibody

AAAS Mouse mAb

#5402

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Western blotting analysis using aladin WD repeat nucleoporin antibody (Cat#5402). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with aladin WD repeat nucleoporin antibody (Cat#5402, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with Aladin WD repeat nucleoporin antibody (Cat#5402, 1:1,000). Nuclei were stained blue with DAPI; Aladin WD repeat nucleoporin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar: 20 μm.
Flow cytometric analysis of Aladin WD repeat nucleoporin expression in HAP-1 cells using Aladin WD repeat nucleoporin antibody (Cat#5402, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Aladin WD repeat nucleoporin.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    AAAS
  • Catalog：
    5402
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name AAAS Mouse mAb
Aliases AAAS; Aladin WD Repeat Nucleoporin; Adracalin; Aladin; Achalasia, Adrenocortical Insufficiency, Alacrimia; Allgrove, Triple-A; ADRACALA; Achalasia, Adrenocortical Insufficiency, Alacrimia (Allgrove, Triple-A); AAASb; GL003; AAA
Background

Gene Name: AAAS

NCBI Gene Entry: 8086

UniProt Entry: Q9NRG9

Application Information

Molecular Weight: Predicted, 60 kDa; observed, 60 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB1340

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human AAAS
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:100-1:1,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using aladin WD repeat nucleoporin antibody (Cat#5402). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with aladin WD repeat nucleoporin antibody (Cat#5402, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HAP-1 cells with Aladin WD repeat nucleoporin antibody (Cat#5402, 1:1,000). Nuclei were stained blue with DAPI; Aladin WD repeat nucleoporin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar: 20 μm.
  • Flow cytometric analysis of Aladin WD repeat nucleoporin expression in HAP-1 cells using Aladin WD repeat nucleoporin antibody (Cat#5402, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Aladin WD repeat nucleoporin.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    AAAS
  • Catalog：
    5402
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using aladin WD repeat nucleoporin antibody (Cat#5402). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with aladin WD repeat nucleoporin antibody (Cat#5402, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with Aladin WD repeat nucleoporin antibody (Cat#5402, 1:1,000). Nuclei were stained blue with DAPI; Aladin WD repeat nucleoporin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar: 20 μm.
Flow cytometric analysis of Aladin WD repeat nucleoporin expression in HAP-1 cells using Aladin WD repeat nucleoporin antibody (Cat#5402, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Aladin WD repeat nucleoporin.
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