KD-Validated Helicase like transcription factor Mouse mAb - Product

KD-Validated Helicase like transcription factor Mouse mAb

#62079

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shRNA Knockdown Validated

Western blotting analysis using HLTF antibody (Cat#62079). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HLTF antibody (Cat#62079, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using HLTF antibody (Cat#62079). HLTF expression in wild-type (WT) and HLTF shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with HLTF antibody (Cat#62079, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

HLTF
Catalog：

62079
Application：

WB
Species Reactivity：

H, M

Information

Data

Information

Product Name	KD-Validated Helicase like transcription factor Mouse mAb
Aliases	HLTF; Helicase Like Transcription Factor; HIP116A; RNF80; SMARCA3; SNF2L3; HLTF1; SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily A, Member 3; SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Member 3; RING-Type E3 Ubiquitin Transferase HLTF; Sucrose Nonfermenting Protein 2-Like 3; Helicase-Like Transcription Factor; RING Finger Protein 80; HIP116; ZBU1; DNA-Binding Protein/Plasminogen Activator Inhibitor-1 Regulator; DNA-Binding Protein/Plasminogen Activator Inhibitor 1 Regulator; Sucrose Nonfermenting-Like 3; SNF2-Like 3; EC 2.3.2.27; EC 3.6.4.-; EC 3.6.1
Background	Gene Name: HLTF NCBI Gene Entry: 6596 UniProt Entry: Q14527
Application Information	Molecular Weight: Predicted, 114 kDa; observed, 114 kDa Clonality: Mouse monoclonal antibody Clone ID: 25GB9855 Species Reactivity: Human, mouse Applications Tested: Western Blotting (WB)
Immunogen	Recombinant protein of human HLTF
Isotype	Mouse IgG1
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:500-1:2,000
Note	This product is for research use only.

Data

Western blotting analysis using HLTF antibody (Cat#62079). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HLTF antibody (Cat#62079, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using HLTF antibody (Cat#62079). HLTF expression in wild-type (WT) and HLTF shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with HLTF antibody (Cat#62079, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

HLTF
Catalog：

62079
Application：

WB
Species Reactivity：

H, M

Datasheet

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