| Product Name |
His Tag Mouse mAb |
| Aliases |
6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag |
| Background |
The His-Tag (or Polyhistidine tag) is a short amino acid sequence, typically composed of six to ten consecutive histidine residues (e.g., His6), genetically engineered onto either the N- or C-terminus of a recombinant protein. Its primary function is to facilitate the purification of the tagged protein using immobilized metal affinity chromatography (IMAC). Histidine residues possess an imidazole side chain that exhibits high affinity for certain divalent metal ions, such as nickel (Ni2+), cobalt (Co2+), copper (Cu2+), or zinc (Zn2+). This specific interaction allows the His-tagged protein to bind tightly to a chromatography resin charged with one of these metal ions. |
| Application Information |
Molecular Weight: Recombinant protein dependent
Clonality: Mouse monoclonal antibody
Clone ID: 25GB7060
Species Reactivity: Recombinant protein
Applications Tested: Western blotting (WB)
|
| Immunogen |
A synthesized peptide derived from His-Tag |
| Isotype |
Mouse IgG1 |
| Storage Buffer |
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide. |
| Storage |
Store at -20 °C for 1 year. |
| Recommended Dilutions |
Western Blotting (WB): 1:1,000-1:5,000 |
| Note |
This product is for research use only. |
