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EIF2B3 Mouse mAb

#5409

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Western blotting analysis using EIF2B3 antibody (Cat#5409). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EIF2B3 antibody (Cat#5409, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of EIF2B3 expression in C2C12 cells using EIF2B3 antibody (Cat#5409, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, EIF2B3.
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Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    EIF2B3
  • Catalog:
    5409
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M, R
Information
Product Name EIF2B3 Mouse mAb
Aliases EIF2B3; Eukaryotic Translation Initiation Factor 2B Subunit Gamma; EIF2Bgamma; EIF-2B; Eukaryotic Translation Initiation Factor 2B, Subunit 3 Gamma, 58kDa; Translation Initiation Factor EIF2B Subunit Gamma; EIF2B GDP-GTP Exchange Factor Subunit Gamma; Eukaryotic Translation Initiation Factor 2B, Subunit 3 (Gamma, 58kD); Translation Initiation Factor EIF-2B Subunit Gamma; EIF-2B GDP-GTP Exchange Factor Subunit Gamma; VWM3
Background

Gene Name: EIF2B3

NCBI Gene Entry: 8891

UniProt Entry: Q9NR50

Application Information

Molecular Weight: Predicted, 50 kDa; observed, 50 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB1375

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen Recombinant protein of human EIF2B3
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using EIF2B3 antibody (Cat#5409). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EIF2B3 antibody (Cat#5409, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of EIF2B3 expression in C2C12 cells using EIF2B3 antibody (Cat#5409, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, EIF2B3.
Western blotting analysis using EIF2B3 antibody (Cat#5409). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EIF2B3 antibody (Cat#5409, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of EIF2B3 expression in C2C12 cells using EIF2B3 antibody (Cat#5409, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, EIF2B3.
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