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FLII Recombinant Rabbit mAb

#5522

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Recombinant Antibody
Western blotting analysis using FLII antibody (Cat#5522). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FLII antibody (Cat#5522, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of FLII expression in HepG2 cells using FLII antibody (Cat#5522, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, FLII.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    FLII
  • Catalog:
    5522
  • Application:
    WB, FCM
  • Species Reactivity:
    H
Information
Product Name FLII Recombinant Rabbit mAb
Aliases FLII; FLII Actin Remodeling Protein; FLIL; Fli1; FLI; Flightless I Actin Binding Protein; Protein Flightless-1 Homolog; MGC39265; Flightless I (Drosophila) Homolog; Flightless I Homolog (Drosophila); Flightless I Homolog; CMD2J
Background

Gene Name: FLII

NCBI Gene Entry: 2314

UniProt Entry: Q13045

Application Information

Molecular Weight: Predicted, 145 kDa; observed, 150 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB1890

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human Flightless 1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using FLII antibody (Cat#5522). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FLII antibody (Cat#5522, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of FLII expression in HepG2 cells using FLII antibody (Cat#5522, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, FLII.
Western blotting analysis using FLII antibody (Cat#5522). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FLII antibody (Cat#5522, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of FLII expression in HepG2 cells using FLII antibody (Cat#5522, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, FLII.
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