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MAP4K4 Recombinant Rabbit mAb

#5523

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Recombinant Antibody
Western blotting analysis using MAP4K4 antibody (Cat#5523). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAP4K4 antibody (Cat#5523, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of H9C2 cells with MAP4K4 antibody (Cat#5523, 1:1,000). Nuclei were stained blue with DAPI; MAP4K4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of MAP4K4 expression in C2C12 cells using MAP4K4 antibody (Cat#5523, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, MAP4K4.
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Unit price:
$69.00
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1
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  • Gene Symbol:
    MAP4K4
  • Catalog:
    5523
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H, M, R
Information
Product Name MAP4K4 Recombinant Rabbit mAb
Aliases MAP4K4; Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4; HGK; NIK; FLH21957; Hepatocyte Progenitor Kinase-Like/Germinal Center Kinase-Like Kinase; MAPK/ERK Kinase Kinase Kinase 4; HPK/GCK-Like Kinase HGK; Nck-Interacting Kinase; MEK Kinase Kinase 4; EC 2.7.11.1; Epididymis Secretory Protein Li 31; Ste20 Group Protein Kinase HGK; HPK/GCK-Like Kinase; EC 2.7.11; HEL-S-31; KIAA0687; MEKKK 4; MEKKK4
Background

Gene Name: MAP4K4

NCBI Gene Entry: 9448

UniProt Entry: O95819

Application Information

Molecular Weight: Predicted, 142 kDa; observed, 140-200 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB1895

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human MAP4K4
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using MAP4K4 antibody (Cat#5523). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAP4K4 antibody (Cat#5523, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of H9C2 cells with MAP4K4 antibody (Cat#5523, 1:1,000). Nuclei were stained blue with DAPI; MAP4K4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of MAP4K4 expression in C2C12 cells using MAP4K4 antibody (Cat#5523, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, MAP4K4.
Western blotting analysis using MAP4K4 antibody (Cat#5523). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAP4K4 antibody (Cat#5523, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of H9C2 cells with MAP4K4 antibody (Cat#5523, 1:1,000). Nuclei were stained blue with DAPI; MAP4K4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of MAP4K4 expression in C2C12 cells using MAP4K4 antibody (Cat#5523, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, MAP4K4.
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