Western blotting analysis using Hsp27 antibody (Cat#5525). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Hsp27 antibody (Cat#5525, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HACAT cells with Hsp27 antibody (Cat#5525, 1:1,000). Nuclei were stained blue with DAPI; Hsp27 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Hsp27 expression in HaCaT cells using Hsp27 antibody (Cat#5525,1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Hsp27.
HSPB1; Heat Shock Protein Family B (Small) Member 1; HSP27; HSP28; Hs.76067; Hsp25; CMT2F; Heat Shock Protein Family B Member 1; Estrogen-Regulated 24 KDa Protein; Stress-Responsive Protein 27; Heat Shock 27kDa Protein 1; Heat Shock 27kD Protein 1; Heat Shock Protein Beta-1; 28 KDa Heat Shock Protein; Heat Shock 27 KDa Protein; SRP27; Epididymis Secretory Protein Li 102; HEL-S-102; HSP 27; HMN2B; HMND3; HspB1
Western blotting analysis using Hsp27 antibody (Cat#5525). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Hsp27 antibody (Cat#5525, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HACAT cells with Hsp27 antibody (Cat#5525, 1:1,000). Nuclei were stained blue with DAPI; Hsp27 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Hsp27 expression in HaCaT cells using Hsp27 antibody (Cat#5525,1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Hsp27.
Western blotting analysis using Hsp27 antibody (Cat#5525). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Hsp27 antibody (Cat#5525, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HACAT cells with Hsp27 antibody (Cat#5525, 1:1,000). Nuclei were stained blue with DAPI; Hsp27 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Hsp27 expression in HaCaT cells using Hsp27 antibody (Cat#5525,1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Hsp27.
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