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Home>Product>Primary Antibody

PPP1CC Mouse mAb

#5551

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Western blotting analysis using PPP1CC antibody (Cat#5551). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PPP1CC antibody (Cat#5551, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with PPP1CC antibody(Cat#5551, 1:1,000). Nuclei were stained blue with DAPI; PPP1CC was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of PPP1CC expression in HAP-1 cells using PPP1CC antibody (Cat#5551, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PPP1CC.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PPP1CC
  • Catalog：
    5551
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name PPP1CC Mouse mAb
Aliases PPP1CC; Protein Phosphatase 1 Catalytic Subunit Gamma; PP1C; Serine/Threonine-Protein Phosphatase PP1-Gamma Catalytic Subunit; Protein Phosphatase 1, Catalytic Subunit, Gamma Isozyme; Protein Phosphatase 1C Catalytic Subunit; EC 3.1.3.16; PP1gamma; PP-1G; Protein Phosphatase 1, Catalytic Subunit, Gamma Isoform; Serine/Threonine Phosphatase 1 Gamma; PPP1G
Background

Gene Name: PPP1CC

NCBI Gene Entry: 5501

UniProt Entry: P36873

Application Information

Molecular Weight: Predicted, 37 kDa; observed, 35 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB4215

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human PPP1CC
Isotype Mouse IgG2b
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using PPP1CC antibody (Cat#5551). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PPP1CC antibody (Cat#5551, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HAP-1 cells with PPP1CC antibody(Cat#5551, 1:1,000). Nuclei were stained blue with DAPI; PPP1CC was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of PPP1CC expression in HAP-1 cells using PPP1CC antibody (Cat#5551, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PPP1CC.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PPP1CC
  • Catalog：
    5551
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using PPP1CC antibody (Cat#5551). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PPP1CC antibody (Cat#5551, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with PPP1CC antibody(Cat#5551, 1:1,000). Nuclei were stained blue with DAPI; PPP1CC was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of PPP1CC expression in HAP-1 cells using PPP1CC antibody (Cat#5551, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PPP1CC.
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