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Home>Product>Primary Antibody

KO-Validated HLA-F Recombinant Rabbit mAb

#71219

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Recombinant Antibody
Knockout Validated
Western blotting analysis using HLA-F antibody (Cat#71219). HLA-F expression in wild-type (WT) and HLA-F knockout (KO) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with HLA-F antibody (Cat#71219, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells with HLA-F antibody (Cat#71219, 1:1,000). Nuclei were stained blue with DAPI; HLA-F was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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  • Gene Symbol：
    HLA-F
  • Catalog：
    71219
  • Application：
    WB, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name KO-Validated HLA-F Recombinant Rabbit mAb
Aliases HLA-F; Major Histocompatibility Complex, Class I, F; HLA Class I Histocompatibility Antigen, Alpha Chain F; MHC Class I Antigen F; Leukocyte Antigen F; HLA F Antigen; HLA-5.4; CDA12; HLAF; Non-Classical MHC Class I Antigen; Nonclassical MHC Class I Antigen; HLA-CDA12
Background

Gene Name: HLA-F

NCBI Gene Entry: 3134

UniProt Entry: P30511

Application Information

Molecular Weight: Predicted, 39 kDa; observed, 42 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB3165

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human HLA F
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using HLA-F antibody (Cat#71219). HLA-F expression in wild-type (WT) and HLA-F knockout (KO) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with HLA-F antibody (Cat#71219, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HeLa cells with HLA-F antibody (Cat#71219, 1:1,000). Nuclei were stained blue with DAPI; HLA-F was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HLA-F
  • Catalog：
    71219
  • Application：
    WB, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using HLA-F antibody (Cat#71219). HLA-F expression in wild-type (WT) and HLA-F knockout (KO) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with HLA-F antibody (Cat#71219, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells with HLA-F antibody (Cat#71219, 1:1,000). Nuclei were stained blue with DAPI; HLA-F was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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