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SIRPA Recombinant Rabbit mAb

#5658

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Recombinant Antibody
Western blotting analysis using SIRPA antibody (Cat#5658). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SIRPA antibody (Cat#5658, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of SIRPA expression in HT-1080 cells using SIRPA antibody (Cat#5658, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, SIRPA.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    SIRPA
  • Catalog:
    5658
  • Application:
    WB, FCM
  • Species Reactivity:
    H
Information
Product Name SIRPA Recombinant Rabbit mAb
Aliases SIRPA; Signal Regulatory Protein Alpha; SHPS1; SIRP; BIT; P84; MFR; SHPS-1; CD172a; PTPNS1; MYD-1; Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1; CD172 Antigen-Like Family Member A; Inhibitory Receptor SHPS-1; Macrophage Fusion Receptor; SIRP-ALPHA-1; SIRPalpha2; SIRPalpha; Brain-Immunoglobulin-Like Molecule With Tyrosine-Based Activation Motifs; Brain Ig-Like Molecule With Tyrosine-Based Activation Motifs; Protein Tyrosine Phosphatase, Non-Receptor Type Substrate 1; Tyrosine Phosphatase SHP Substrate 1; Signal-Regulatory Protein Alpha-1; Signal-Regulatory Protein Alpha-2; Signal-Regulatory Protein Alpha-3; Signal-Regulatory Protein Alpha; SHP Substrate 1; CD172a Antigen; Myd-1 Antigen; MyD-1 Antigen; Sirp-Alpha-1; irp-Alpha-2; Sirp-Alpha-3; MYD1; Bit
Background

Gene Name: SIRPA

NCBI Gene Entry: 140885

UniProt Entry: P78324

Application Information

Molecular Weight: Predicted, 55 kDa; observed, 65-85 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB4740

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen Recombinant protein of human SIRPA
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using SIRPA antibody (Cat#5658). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SIRPA antibody (Cat#5658, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of SIRPA expression in HT-1080 cells using SIRPA antibody (Cat#5658, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, SIRPA.
Western blotting analysis using SIRPA antibody (Cat#5658). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SIRPA antibody (Cat#5658, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of SIRPA expression in HT-1080 cells using SIRPA antibody (Cat#5658, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, SIRPA.
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