Western blotting analysis using DNAJB1 antibody (Cat#5758). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DNAJB1 antibody (Cat#5758, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of DNAJB1 expression in HepG2 cells using DNAJB1 antibody (Cat#5758, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, DNAJB1.
DNAJB1; DnaJ Heat Shock Protein Family (Hsp40) Member B1; RSPH16B; Hsp40; HSPF1; Sis1; DnaJ (Hsp40) Homolog, Subfamily B, Member 1; DnaJ Homolog Subfamily B Member 1; Heat Shock 40 KDa Protein 1; DnaJ Protein Homolog 1; Human DnaJ Protein 1; Radial Spoke 16 Homolog B (Chlamydomonas); Radial Spoke 16 Homolog B; Heat Shock Protein 40; DNAJ1; HSP40; HDj-1; Hdj1; HDJ1
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human DNAJB1
Isotype
Mouse IgG2b
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using DNAJB1 antibody (Cat#5758). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DNAJB1 antibody (Cat#5758, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of DNAJB1 expression in HepG2 cells using DNAJB1 antibody (Cat#5758, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, DNAJB1.
Western blotting analysis using DNAJB1 antibody (Cat#5758). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DNAJB1 antibody (Cat#5758, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of DNAJB1 expression in HepG2 cells using DNAJB1 antibody (Cat#5758, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, DNAJB1.
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