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Home>Product>Primary Antibody

PSMA6 Mouse mAb

#5765

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Western blotting analysis using PSMA6 antibody (Cat#5765). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PSMA6 antibody (Cat#5765, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with PSMA6 antibody (Cat#5765, 1:1,000). Nuclei were stained blue with DAPI; PSMA6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of PSMA6 expression in HepG2 cells using PSMA6 antibody (Cat#5765, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PSMA6.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PSMA6
  • Catalog：
    5765
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name PSMA6 Mouse mAb
Aliases PSMA6; Proteasome 20S Subunit Alpha 6; PROS27; P27K; IOTA; Proteasome (Prosome, Macropain) Subunit, Alpha Type, 6; Multicatalytic Endopeptidase Complex Iota Chain; Proteasome Subunit Alpha Type-6; Proteasome Subunit Alpha 6; 27 KDa Prosomal Protein; Proteasome Iota Chain; Macropain Iota Chain; MGC22756; MGC23846; MGC2333; PROS-27; Testicular Secretory Protein Li 44; Proteasome Subunit Alpha1; Proteasome Subunit Iota; Macropain Subunit Iota; Proteasome Subunit Α1; Prosomal P27K Protein; EC 3.4.25.1
Background

Gene Name: PSMA6

NCBI Gene Entry: 5687

UniProt Entry: P60900

Application Information

Molecular Weight: Predicted, 27 kDa; observed, 27 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB5275

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human PSMA6
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using PSMA6 antibody (Cat#5765). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PSMA6 antibody (Cat#5765, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with PSMA6 antibody (Cat#5765, 1:1,000). Nuclei were stained blue with DAPI; PSMA6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of PSMA6 expression in HepG2 cells using PSMA6 antibody (Cat#5765, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PSMA6.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PSMA6
  • Catalog：
    5765
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using PSMA6 antibody (Cat#5765). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PSMA6 antibody (Cat#5765, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with PSMA6 antibody (Cat#5765, 1:1,000). Nuclei were stained blue with DAPI; PSMA6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of PSMA6 expression in HepG2 cells using PSMA6 antibody (Cat#5765, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PSMA6.
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