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Home>Product>Primary Antibody

SERPINA1 Mouse mAb

#6553

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Western blotting analysis using SERPINA1 antibody (Cat#6553). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SERPINA1 antibody (Cat#6553, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using SERPINA1 antibody (Cat#6553, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
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  • Gene Symbol：
    SERPINA1
  • Catalog：
    6553
  • Application：
    WB, IHC-P
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name SERPINA1 Mouse mAb
Aliases SERPINA1; Serpin Family A Member 1; Alpha-1-Antitrypsin; AAT; Alpha1AT; A1AT; A1A; PI1; PI; Serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1; Alpha-1 Proteinase Inhibitor; Alpha-1 Protease Inhibitor; Alpha-1-Antiproteinase; Alpha-1 Antitrypsin; Anti-Elastase; Serpin A1; Serine (Or Cysteine) Proteinase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1; Serpin Peptidase Inhibitor Clade A (Alpha-1antiproteinase, Antitrypsin) Member 1; Serine (Or Cysteine) Proteinase Inhibitor, Clade A, Member 1; Protease Inhibitor 1 (Anti-Elastase), Alpha-1-Antitrypsin; Serpin Peptidase Inhibitor Clade A Member 1; Epididymis Secretory Sperm Binding Protein; Protease Inhibitor 1; PRO2275; NNIF
Background

Gene Name: SERPINA1

NCBI Gene Entry: 5265

UniProt Entry: P01009

Application Information

Molecular Weight: Predicted, 47 kDa; observed, 55 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB9990

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P)
Immunogen Recombinant protein of human SERPINA1
Isotype Mouse IgG2b
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.03% Proclin 300.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Immunohistochemistry-Paraffin (IHC-P): 1:50-1:100
Note This product is for research use only.
Data
  • Western blotting analysis using SERPINA1 antibody (Cat#6553). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SERPINA1 antibody (Cat#6553, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using SERPINA1 antibody (Cat#6553, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SERPINA1
  • Catalog：
    6553
  • Application：
    WB, IHC-P
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using SERPINA1 antibody (Cat#6553). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SERPINA1 antibody (Cat#6553, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using SERPINA1 antibody (Cat#6553, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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