Western blotting analysis using IL13 receptor alpha 1 antibody (Cat#62240). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IL13 receptor alpha 1 antibody (Cat#62240, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using il13 receptor α 1 antibody (Cat#62240). IL13 receptor α 1 expression in wild type (WT) and il13 receptor α 1 (IL13RA1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with il13 receptor α 1 antibody (Cat#62240, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of IL13 receptor alpha 1 expression in HepG2 cells using IL13 receptor alpha 1 antibody (Cat#62240, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, IL13 receptor alpha 1.
Validation of IL13 receptor alpha 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with IL13 receptor alpha 1 antibody (Cat#62240, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
