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Home>Product>Primary Antibody

MYH9 Mouse mAb

#5773

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Western blotting analysis using MYH9 antibody (Cat#5773). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MYH9 antibody (Cat#5773, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human renal carcinoma using MYH9 antibody (Cat#5773, 1:1,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MYH9
  • Catalog：
    5773
  • Application：
    WB, IHC-P
  • Species Reactivity：
    H

Information

Data

Information
Product Name MYH9 Mouse mAb
Aliases MYH9; Myosin Heavy Chain 9; NMHC-II-A; NMMHCA; EPSTS; FTNS; MHA; Cellular Myosin Heavy Chain, Type A; Nonmuscle Myosin Heavy Chain II-A; Non-Muscle Myosin Heavy Chain IIa; Non-Muscle Myosin Heavy Chain A; MMHC-IIA; Myosin-9; DFNA17; Myosin, Heavy Polypeptide 9, Non-Muscle; Non-Muscle Myosin Heavy Polypeptide 9; Myosin Heavy Chain, Non-Muscle IIa; Myosin, Heavy Chain 9, Non-Muscle; Non-Muscle Myosin Heavy Chain 9; Nonmuscle Myosin IIA2; NMMHC II-A; NMMHC-A; BDPLT6; MATINS
Background

Gene Name: MYH9

NCBI Gene Entry: 4627

UniProt Entry: P35579

Application Information

Molecular Weight: Predicted, 227 kDa; observed, 227 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB5315

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P)
Immunogen Recombinant protein of human MYH9
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Immunohistochemistry-Paraffin (IHC-P): 1:500-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using MYH9 antibody (Cat#5773). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MYH9 antibody (Cat#5773, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human renal carcinoma using MYH9 antibody (Cat#5773, 1:1,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MYH9
  • Catalog：
    5773
  • Application：
    WB, IHC-P
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using MYH9 antibody (Cat#5773). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MYH9 antibody (Cat#5773, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human renal carcinoma using MYH9 antibody (Cat#5773, 1:1,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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