Cart

Home

About

Standards

Product

Custom Services

Signal Pathways

Technical Support

News

Sign In/Register

Cart
  • Primary Antibody
  • Secondary Antibodies
  • Lentiviral Particles
  • Stable Cell Lines
  • Lysates
  • Reagents
  • Other Products
Home>Product>Primary Antibody

Sirtuin 5 Mouse mAb

#5784

Click to copy product information

Return to List

Western blotting analysis using sirtuin 5 antibody (Cat#5784). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with sirtuin 5 antibody (Cat#5784, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Sirtuin 5 antibody (Cat#5784, 1:1,000). Nuclei were stained blue with DAPI; Sirtuin 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Sirtuin 5 expression in HepG2 cells using Sirtuin 5 antibody (Cat#5784, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Sirtuin 5.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SIRT5
  • Catalog：
    5784
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name Sirtuin 5 Mouse mAb
Aliases SIRT5; Sirtuin 5; NAD-Dependent Protein Deacylase Sirtuin-5, Mitochondrial; SIR2L5; Sirtuin (Silent Mating Type Information Regulation 2 Homolog) 5 (S. Cerevisiae); Sirtuin (Silent Mating Type Information Regulation 2, S.Cerevisiae, Homolog) 5; NAD-Dependent Lysine Demalonylase And Desuccinylase Sirtuin-5, Mitochondrial; Silent Mating Type Information Regulation 2, S.Cerevisiae, Homolog 5; NAD-Dependent Deacetylase Sirtuin-5; Regulatory Protein SIR2 Homolog 5; SIR2-Like Protein 5; Sirtuin Type 5; Sir2-Like 5; EC 2.3.1.-
Background

Gene Name: SIRT5

NCBI Gene Entry: 23408

UniProt Entry: Q9NXA8

Application Information

Molecular Weight: Predicted, 34 kDa; observed, 30 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB5370

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human SIRT5
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using sirtuin 5 antibody (Cat#5784). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with sirtuin 5 antibody (Cat#5784, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with Sirtuin 5 antibody (Cat#5784, 1:1,000). Nuclei were stained blue with DAPI; Sirtuin 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Sirtuin 5 expression in HepG2 cells using Sirtuin 5 antibody (Cat#5784, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Sirtuin 5.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SIRT5
  • Catalog：
    5784
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using sirtuin 5 antibody (Cat#5784). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with sirtuin 5 antibody (Cat#5784, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Sirtuin 5 antibody (Cat#5784, 1:1,000). Nuclei were stained blue with DAPI; Sirtuin 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Sirtuin 5 expression in HepG2 cells using Sirtuin 5 antibody (Cat#5784, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Sirtuin 5.
Shopping cart ()
Estimated total price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$
View cart Empty cart
  • Phone Number

    +1 (301) 545-2395

  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

微信公众号

Copyright © 2025 Hefei GenuIN Biotech Co., Ltd.

All Rights Reserved.