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Home>Product>Primary Antibody

PDLIM7 Recombinant Rabbit mAb

#5823

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Recombinant Antibody
Western blotting analysis using PDLIM7 antibody (Cat#5823). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PDLIM7 antibody (Cat#5823, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with PDLIM7 antibody (Cat#5823, 1:1,000). Nuclei were stained blue with DAPI; PDLIM7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of PDLIM7 expression in HepG2 cells using PDLIM7 antibody (Cat#5823, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PDLIM7.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PDLIM7
  • Catalog：
    5823
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name PDLIM7 Recombinant Rabbit mAb
Aliases PDLIM7; PDZ And LIM Domain 7; ENIGMA; PDZ And LIM Domain 7 (Enigma); PDZ And LIM Domain Protein 7; Protein Enigma; LMP; Lim Mineralization Protein 3; LIM Mineralization Protein; LIM Domain Protein; 1110003B01Rik; LMP1; LMP3
Background

Gene Name: PDLIM7

NCBI Gene Entry: 9260

UniProt Entry: Q9NR12

Application Information

Molecular Weight: Predicted, 50 kDa; observed, 49 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB5570

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human PDLIM7
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using PDLIM7 antibody (Cat#5823). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PDLIM7 antibody (Cat#5823, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with PDLIM7 antibody (Cat#5823, 1:1,000). Nuclei were stained blue with DAPI; PDLIM7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of PDLIM7 expression in HepG2 cells using PDLIM7 antibody (Cat#5823, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PDLIM7.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PDLIM7
  • Catalog：
    5823
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using PDLIM7 antibody (Cat#5823). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PDLIM7 antibody (Cat#5823, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with PDLIM7 antibody (Cat#5823, 1:1,000). Nuclei were stained blue with DAPI; PDLIM7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of PDLIM7 expression in HepG2 cells using PDLIM7 antibody (Cat#5823, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PDLIM7.
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