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PSMC3 Mouse mAb

#5833

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Western blotting analysis using PSMC3 antibody (Cat#5833). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PSMC3 antibody (Cat#5833, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of PSMC3 expression in HT-1080 cells using PSMC3 antibody (Cat#5833, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PSMC3.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    PSMC3
  • Catalog:
    5833
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M, R
Information
Product Name PSMC3 Mouse mAb
Aliases PSMC3; Proteasome 26S Subunit, ATPase 3; TBP1; TBP-1; RPT5; Proteasome (Prosome, Macropain) 26S Subunit, ATPase, 3; 26S Proteasome AAA-ATPase Subunit RPT5; 26S Proteasome Regulatory Subunit 6A; Proteasome Subunit P50; Tat-Binding Protein 1; Human Immunodeficiency Virus Tat Transactivator Binding Protein-1; 26S Protease Regulatory Subunit 6A; Testicular Secretory Protein Li 42; Proteasome 26S Subunit ATPase; Tat-Binding Protein-1; DCIDP
Background

Gene Name: PSMC3

NCBI Gene Entry: 5702

UniProt Entry: P17980

Application Information

Molecular Weight: Predicted, 49 kDa; observed, 49 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB5060

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen Recombinant protein of human PSMC3
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using PSMC3 antibody (Cat#5833). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PSMC3 antibody (Cat#5833, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of PSMC3 expression in HT-1080 cells using PSMC3 antibody (Cat#5833, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PSMC3.
Western blotting analysis using PSMC3 antibody (Cat#5833). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PSMC3 antibody (Cat#5833, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of PSMC3 expression in HT-1080 cells using PSMC3 antibody (Cat#5833, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PSMC3.
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