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MARS1 Recombinant Rabbit mAb

#6041

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Recombinant Antibody
Western blotting analysis using MARS1 antibody (Cat#6041). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MARS1 antibody (Cat#6041, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human ovarian carcinoma using MARS1 antibody (Cat#6041, 1:1,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    MARS1
  • Catalog:
    6041
  • Application:
    WB, IHC-P
  • Species Reactivity:
    H, R
Information
Product Name MARS1 Recombinant Rabbit mAb
Aliases MARS1; Methionyl-TRNA Synthetase 1; MetRS; Methionine--TRNA Ligase, Cytoplasmic; SPG70; CMT2U; MARS; Methionyl-TRNA Synthetase; EC 6.1.1.10; Methionine TRNA Ligase 1, Cytoplasmic; Cytosolic Methionyl-TRNA Synthetase; ILFS2; MTRNS; ILLD; TTD9; MRS
Background

Gene Name: MARS1

NCBI Gene Entry: 4141

UniProt Entry: P56192

Application Information

Molecular Weight: Predicted, 101 kDa; observed, 95 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB6530

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P)

Immunogen A synthesized peptide derived from human MARS
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunohistochemistry-Paraffin (IHC-P): 1:500-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using MARS1 antibody (Cat#6041). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MARS1 antibody (Cat#6041, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human ovarian carcinoma using MARS1 antibody (Cat#6041, 1:1,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Western blotting analysis using MARS1 antibody (Cat#6041). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MARS1 antibody (Cat#6041, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human ovarian carcinoma using MARS1 antibody (Cat#6041, 1:1,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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