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Home>Product>Primary Antibody

ATG4A Recombinant Rabbit mAb

#1583

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Recombinant Antibody
Western blotting analysis using ATG4A antibody (Cat#1583). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATG4A antibody (Cat#1583, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with ATG4A antibody (Cat#1583, 1:1,000). Nuclei were stained blue with DAPI; ATG4A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ATG4A expression in C2C12 cells using ATG4A antibody (Cat#1583, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ATG4A.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ATG4A
  • Catalog：
    1583
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name ATG4A Recombinant Rabbit mAb
Aliases ATG4A; Autophagy Related 4A Cysteine Peptidase; APG4A; AUTL2; Autophagy-Related Cysteine Endopeptidase; Autophagy-Related Protein 4 Homolog A; Cysteine Protease ATG4A; HsAPG4A; HAPG4A; AUT-Like 2, Cysteine Endopeptidase (S. Cerevisiae); ATG4 Autophagy Related 4 Homolog A (S. Cerevisiae); APG4 Autophagy 4 Homolog A (S. Cerevisiae); Autophagy Related 4A, Cysteine Peptidase; ATG4 Autophagy Related 4 Homolog A; AUT-Like 2, Cysteine Endopeptidase; AUT-Like 2 Cysteine Endopeptidase; APG4 Autophagy 4 Homolog A; Autophagin; Autophagin-2; EC 3.4.22.-; EC 3.4.22
Background

Gene Name: ATG4A

NCBI Gene Entry: 115201

UniProt Entry: Q8WYN0

Application Information

Molecular Weight: Predicted, 45 kDa; observed, 45 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB3235

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Atg4A
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using ATG4A antibody (Cat#1583). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATG4A antibody (Cat#1583, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of C2C12 cells with ATG4A antibody (Cat#1583, 1:1,000). Nuclei were stained blue with DAPI; ATG4A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of ATG4A expression in C2C12 cells using ATG4A antibody (Cat#1583, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ATG4A.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ATG4A
  • Catalog：
    1583
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using ATG4A antibody (Cat#1583). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATG4A antibody (Cat#1583, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with ATG4A antibody (Cat#1583, 1:1,000). Nuclei were stained blue with DAPI; ATG4A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ATG4A expression in C2C12 cells using ATG4A antibody (Cat#1583, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ATG4A.
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