Western blotting analysis using nuclear factor kappa B subunit 2 antibody (Cat#61602). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with nuclear factor kappa B subunit 2 antibody (Cat#61602, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using nuclear factor kappa B subunit 2 antibody (Cat#61602). Nuclear factor kappa B subunit 2 expression in wild-type (WT) and nuclear factor kappa B subunit 2 (NFKB2) knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with nuclear factor kappa B subunit 2 antibody (Cat#61602, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with Nuclear factor kappa B subunit 2 antibody (Cat#61602, 1:1,000). Nuclei were stained blue with DAPI; Nuclear factor kappa B subunit 2 was stained magenta with Alexa Fluor® 647. lmages were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of Nuclear factor kappa B subunit 2 expression in HepG2 cells using Nuclear factor kappa B subunit 2 antibody (Cat#61602, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Nuclear factor kappa B subunit 2.
