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Home>Product>Primary Antibody

H2B Clustered Histone 12 Recombinant Rabbit mAb

#2949

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Recombinant Antibody
Western blotting analysis using histone H2B antibody (Cat#2949). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with histone H2B antibody (Cat#2949, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using histone H2B antibody (Cat#2949, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with Histone H2B antibody (Cat#2949, 1:1,000). Nuclei were stained blue with DAPI; Histone H2B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
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  • Gene Symbol：
    H2BC12
  • Catalog：
    2949
  • Application：
    WB, ICC, IHC-P
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name H2B Clustered Histone 12 Recombinant Rabbit mAb
Aliases H2BC12; H2B Clustered Histone 12; HIST1H2B; H2BFAiii; H2BFT; Histone Cluster 1 H2B Family Member K; H2B Histone Family, Member T; HIRA-Interacting Protein 1; Histone Cluster 1, H2bk; Histone H2B Type 1-K; Histone 1, H2bk; H2B K; Histone Family Member; HIRIP1; H2B/S; H2BK
Background

Gene Name: H2BC12

NCBI Gene Entry: 85236

UniProt Entry: O60814

Application Information

Molecular Weight: Predicted, 14 kDa; observed, 17 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3315

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human Histone H2B
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:4,000-1:20,000
Immunocytochemistry (ICC): 1:100-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using histone H2B antibody (Cat#2949). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with histone H2B antibody (Cat#2949, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using histone H2B antibody (Cat#2949, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunocytochemical staining of HepG2 cells with Histone H2B antibody (Cat#2949, 1:1,000). Nuclei were stained blue with DAPI; Histone H2B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    H2BC12
  • Catalog：
    2949
  • Application：
    WB, ICC, IHC-P
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using histone H2B antibody (Cat#2949). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with histone H2B antibody (Cat#2949, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using histone H2B antibody (Cat#2949, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with Histone H2B antibody (Cat#2949, 1:1,000). Nuclei were stained blue with DAPI; Histone H2B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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