Western blotting analysis using C-terminal binding protein 2 antibody (Cat#61918). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with C-terminal binding protein 2 antibody (Cat#61918, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using C-terminal binding protein 2 antibody (Cat#61918). C-terminal binding protein 2 expression in wild type (WT) and C-terminal binding protein 2 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with C-terminal binding protein 2 antibody (Cat#61918, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of C-terminal binding protein 2 expression in HeLa cells using C-terminal binding protein 2 antibody (Cat#61918, 1:2,000). Green, isotype control; red, C-terminal binding protein 2.
Immunocytochemical staining of HeLa cells with C-terminal binding protein 2 antibody (Cat #61918, 1:1,000) . Nuclei were stained blue with DAPI; C-terminal binding protein 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded human glioblastoma using c-terminal binding protein 2 antibody (Cat#61918, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
