Western blotting analysis using calpain 9 antibody (Cat#6404). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with calpain 9 antibody (Cat#6404, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:400-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using calpain 9 antibody (Cat#6404). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with calpain 9 antibody (Cat#6404, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using calpain 9 antibody (Cat#6404). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with calpain 9 antibody (Cat#6404, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000958s ]
SELECT * FROM `goods` WHERE `code` = '6404' LIMIT 1 [ RunTime:0.000621s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000641s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000451s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000510s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 10146 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000497s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000608s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 10146 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000344s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000520s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000333s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.136014s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (6718,9762,8548,9372,10240,5885,9987,7790,7363,8011) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.006144s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.001023s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.002228s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000502s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000257s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.003494s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.005133s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000886s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000246s ]
