Western blotting analysis using interferon gamma receptor 1 antibody (Cat#64900). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with interferon gamma receptor 1 antibody (Cat#64900, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using interferon gamma receptor 1 antibody (Cat#64900). Interferon gamma receptor 1 expression in wild-type (WT) and interferon gamma receptor 1 (IFNGR1) shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with interferon gamma receptor 1 antibody (Cat#64900, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Interferon gamma receptor 1 antibody (Cat#64900, 1:1,000). Nuclei were stained blue with DAPI; Interferon gamma receptor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of Interferon gamma receptor 1 expression in C2C12 cells using Interferon gamma receptor 1 antibody (Cat#64900, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Interferon gamma receptor 1.
