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Home>Product>Primary Antibody

SIRT3 Recombinant Rabbit mAb

#6055

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Recombinant Antibody
Western blotting analysis using sirtuin 3 antibody (Cat#6055). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with sirtuin 3 antibody (Cat#6055, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using sirtuin 3 antibody (Cat#6055, 1:500). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SIRT3
  • Catalog：
    6055
  • Application：
    WB, IHC-P
  • Species Reactivity：
    H

Information

Data

Information
Product Name SIRT3 Recombinant Rabbit mAb
Aliases SIRT3; Sirtuin 3; SIR2L3; NAD-Dependent Protein Deacetylase Sirtuin-3, Mitochondrial; Regulatory Protein SIR2 Homolog 3; SIR2-Like Protein 3; Sirtuin (Silent Mating Type Information Regulation 2 Homolog) 3 (S. Cerevisiae); Sirtuin (Silent Mating Type Information Regulation 2, S.Cerevisiae, Homolog) 3; Mitochondrial Nicotinamide Adenine Dinucleotide-Dependent Deacetylase; Silent Mating Type Information Regulation 2, S.Cerevisiae, Homolog 3; NAD-Dependent Deacetylase Sirtuin-3, Mitochondrial; Sirtuin Type 3; EC 2.3.1.286; Sir2-Like 3; HSIRT3
Background

Gene Name: SIRT3

NCBI Gene Entry: 23410

UniProt Entry: Q9NTG7

Application Information

Molecular Weight: Predicted, 44 kDa; observed, 28 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB6600

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human SIRT3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunohistochemistry-Paraffin (IHC-P): 1:250-1:500
Note This product is for research use only.
Data
  • Western blotting analysis using sirtuin 3 antibody (Cat#6055). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with sirtuin 3 antibody (Cat#6055, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using sirtuin 3 antibody (Cat#6055, 1:500). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SIRT3
  • Catalog：
    6055
  • Application：
    WB, IHC-P
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using sirtuin 3 antibody (Cat#6055). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with sirtuin 3 antibody (Cat#6055, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using sirtuin 3 antibody (Cat#6055, 1:500). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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