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NR2F2 Recombinant Rabbit mAb

#6076

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Recombinant Antibody
Western blotting analysis using NR2F2 antibody (Cat#6076). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NR2F2 antibody (Cat#6076, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human endometrial carcinoma using NR2F2 antibody (Cat#6076, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse brown adipose tissue using NR2F2 antibody (Cat#6076, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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Unit price:
$69.00
Quantity:
1
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  • Gene Symbol:
    NR2F2
  • Catalog:
    6076
  • Application:
    WB, IHC-P
  • Species Reactivity:
    H, M, R
Information
Product Name NR2F2 Recombinant Rabbit mAb
Aliases NR2F2; Nuclear Receptor Subfamily 2 Group F Member 2; COUP Transcription Factor II; COUPTFB; COUPTF2; TFCOUP2; SVP40; NF-E3; ARP1; Apolipoprotein A-I Regulatory Protein 1; COUP Transcription Factor 2; COUP-TFII; ARP-1; Chicken Ovalbumin Upstream Promoter Transcription Factor 2; Chicken Ovalbumin Upstream Promoter-Transcription Factor I; Nuclear Receptor Subfamily 2, Group F, Member 2; ADP-Ribosylation Factor Related Protein 1; Apolipoprotein AI Regulatory Protein 1; COUP-TF II; COUPTFII; COUP-TF2; CHTD4; SRXX5
Background

Gene Name: NR2F2

NCBI Gene Entry: 7026

UniProt Entry: P24468

Application Information

Molecular Weight: Predicted, 46 kDa; observed, 46 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB6705

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P)

Immunogen A synthesized peptide derived from human NR2F2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using NR2F2 antibody (Cat#6076). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NR2F2 antibody (Cat#6076, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human endometrial carcinoma using NR2F2 antibody (Cat#6076, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunohistochemistry was performed on paraffin-embedded mouse brown adipose tissue using NR2F2 antibody (Cat#6076, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Western blotting analysis using NR2F2 antibody (Cat#6076). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NR2F2 antibody (Cat#6076, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human endometrial carcinoma using NR2F2 antibody (Cat#6076, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse brown adipose tissue using NR2F2 antibody (Cat#6076, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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