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Cyclin Dependent Kinase Inhibitor 2A Recombinant Rabbit mAb

#3169

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Recombinant Antibody
Western blotting analysis using cyclin dependent kinase inhibitor 2A antibody (Cat#3169). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cyclin dependent kinase inhibitor 2A antibody (Cat#3169, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of Hela cells with Cyclin dependent kinase inhibitor 2A antibody (Cat#3169, 1:1,000). Nuclei were stained blue with DAPI;Cyclin dependent kinase inhibitor 2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High.Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Cyclin dependent kinase inhibitor 2A expression in HeLa cells using Cyclin dependent kinase inhibitor 2A antibody (Cat#3169, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Cyclin dependent kinase inhibitor 2A.
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Unit price:
$69.00
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  • Gene Symbol:
    CDKN2A
  • Catalog:
    3169
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H
Information
Product Name Cyclin Dependent Kinase Inhibitor 2A Recombinant Rabbit mAb
Aliases CDKN2A; Cyclin Dependent Kinase Inhibitor 2A; P14ARF; CDK4I; MTS1; ARF; Cyclin-Dependent Kinase 4 Inhibitor A; P16-INK4A; P16INK4a; P19Arf; INK4a; CDKN2; CMM2; INK4; P16; P19; P14; MLM; Cyclin-Dependent Kinase Inhibitor 2A (Melanoma, P16,Inhibits CDK4); Cyclin-Dependent Kinase Inhibitor 2A; Multiple Tumour Suppressor 1; Multiple Tumor Suppressor 1; CDKN2A/ARF Intron 2 LncRNA; Alternative Reading Frame; Inhibitor Of Cdk4 A; MTS-1; CAI2; Cell Cycle Negative Regulator Beta; P19 Alternate Open Reading Frame; P14 Alternate Open Reading Frame; CDK4 Inhibitor P16-INK4; Tumor Suppressor ARF; P16-INK4a; P16-INK4; P16INK4A; P16INK4; TP16
Background

Gene Name: CDKN2A

NCBI Gene Entry: 1029

UniProt Entry: P42771

Application Information

Molecular Weight: Predicted, 17 kDa; observed, 17 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB4345

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human p16 INK
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using cyclin dependent kinase inhibitor 2A antibody (Cat#3169). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cyclin dependent kinase inhibitor 2A antibody (Cat#3169, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of Hela cells with Cyclin dependent kinase inhibitor 2A antibody (Cat#3169, 1:1,000). Nuclei were stained blue with DAPI;Cyclin dependent kinase inhibitor 2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High.Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Cyclin dependent kinase inhibitor 2A expression in HeLa cells using Cyclin dependent kinase inhibitor 2A antibody (Cat#3169, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Cyclin dependent kinase inhibitor 2A.
Western blotting analysis using cyclin dependent kinase inhibitor 2A antibody (Cat#3169). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cyclin dependent kinase inhibitor 2A antibody (Cat#3169, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of Hela cells with Cyclin dependent kinase inhibitor 2A antibody (Cat#3169, 1:1,000). Nuclei were stained blue with DAPI;Cyclin dependent kinase inhibitor 2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High.Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Cyclin dependent kinase inhibitor 2A expression in HeLa cells using Cyclin dependent kinase inhibitor 2A antibody (Cat#3169, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Cyclin dependent kinase inhibitor 2A.
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