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SMARCD1 Recombinant Rabbit mAb

#6078

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Recombinant Antibody
Western blotting analysis using SMARCD1 antibody (Cat#6078). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMARCD1 antibody (Cat#6078, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded mouse testis tissue using SMARCD1 antibody (Cat#6078, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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Unit price:
$69.00
Quantity:
1
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  • Gene Symbol:
    SMARCD1
  • Catalog:
    6078
  • Application:
    WB, IHC-P
  • Species Reactivity:
    H, M
Information
Product Name SMARCD1 Recombinant Rabbit mAb
Aliases SMARCD1; SWI/SNF Related BAF Chromatin Remodeling Complex Subunit D1; BAF60A; SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily D, Member 1; CRACD1; Rsc6p; SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily D Member 1; 60 KDa BRG-1/Brm-Associated Factor Subunit A; BRG1-Associated Factor 60A; Mammalian Chromatin Remodeling Complex BRG1-Associated Factor 60A; Chromatin Remodeling Complex BAF60A Subunit; SWI/SNF Complex 60 KDa Subunit A; SWI/SNF Complex 60 KDa Subunit; Swp73-Like Protein; CSS11
Background

Gene Name: SMARCD1

NCBI Gene Entry: 6602

UniProt Entry: Q96GM5

Application Information

Molecular Weight: Predicted, 58 kDa; observed, 58 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB6715

Species Reactivity: Human,mouse

Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P)

Immunogen A synthesized peptide derived from human SMARCD1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using SMARCD1 antibody (Cat#6078). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMARCD1 antibody (Cat#6078, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded mouse testis tissue using SMARCD1 antibody (Cat#6078, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Western blotting analysis using SMARCD1 antibody (Cat#6078). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMARCD1 antibody (Cat#6078, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded mouse testis tissue using SMARCD1 antibody (Cat#6078, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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