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Home>Product>Primary Antibody

KD-Validated ROCK2 Recombinant Rabbit mAb

#61147

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using ROCK2 antibody (Cat#61147). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ROCK2 antibody (Cat#61147, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using ROCK2 antibody (Cat#61147). ROCK2 expression in wild type (WT) and ROCK2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ROCK2 antibody (Cat#61147, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with ROCK2 antibody (Cat#61147, 1:1,000). Nuclei were stained blue with DAPI; ROCK2 cochaperone 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Immunocytochemical staining of HeLa cells using ROCK2 antibody (Cat#61147, 1:1,000), Top panel: wild-type (WT); Bottom panal: ROCK2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; ROCK2 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ROCK2 expression in HepG2 cells using ROCK2 antibody (Cat#61147, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ROCK2.
Validation of ROCK2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with ROCK2 antibody (Cat#61147, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ROCK2
  • Catalog：
    61147
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated ROCK2 Recombinant Rabbit mAb
Aliases ROCK2; Rho Associated Coiled-Coil Containing Protein Kinase 2; Rho-Associated, Coiled-Coil-Containing Protein Kinase II; Rho-Associated Protein Kinase 2; P164 ROCK-2; EC 2.7.11.1; ROCK-II; Rho-Associated, Coiled-Coil-Containing Protein Kinase 2; Rho Kinase 2; EC 2.7.11; KIAA0619
Background

Gene Name: ROCK2

NCBI Gene Entry: 9475

UniProt Entry: O75116

Application Information

Molecular Weight: Predicted, 161 kDa; observed, 161 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB2030

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human ROCK2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using ROCK2 antibody (Cat#61147). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ROCK2 antibody (Cat#61147, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using ROCK2 antibody (Cat#61147). ROCK2 expression in wild type (WT) and ROCK2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ROCK2 antibody (Cat#61147, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with ROCK2 antibody (Cat#61147, 1:1,000). Nuclei were stained blue with DAPI; ROCK2 cochaperone 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Immunocytochemical staining of HeLa cells using ROCK2 antibody (Cat#61147, 1:1,000), Top panel: wild-type (WT); Bottom panal: ROCK2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; ROCK2 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of ROCK2 expression in HepG2 cells using ROCK2 antibody (Cat#61147, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ROCK2.
  • Validation of ROCK2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with ROCK2 antibody (Cat#61147, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ROCK2
  • Catalog：
    61147
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using ROCK2 antibody (Cat#61147). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ROCK2 antibody (Cat#61147, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using ROCK2 antibody (Cat#61147). ROCK2 expression in wild type (WT) and ROCK2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ROCK2 antibody (Cat#61147, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with ROCK2 antibody (Cat#61147, 1:1,000). Nuclei were stained blue with DAPI; ROCK2 cochaperone 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Immunocytochemical staining of HeLa cells using ROCK2 antibody (Cat#61147, 1:1,000), Top panel: wild-type (WT); Bottom panal: ROCK2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; ROCK2 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ROCK2 expression in HepG2 cells using ROCK2 antibody (Cat#61147, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ROCK2.
Validation of ROCK2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with ROCK2 antibody (Cat#61147, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
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