KD-Validated F-Box Protein 11 Mouse mAb - Product - Hefei GenuIN Biotech Co., Ltd.

KD-Validated F-Box Protein 11 Mouse mAb

#61262

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Knockdown Validated

Western blotting analysis using FBXO11 antibody (Cat#61262). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FBXO11 antibody (Cat#61262, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using FBXO11 antibody (Cat#61262). FBXO11 expression in wild-type (WT) and FBXO11 knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with FBXO11 antibody (Cat#61262, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Flow cytometric analysis of FBXO11 expression in HeLa cells using FBXO11 antibody (Cat# 61262, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, FBXO11.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

FBXO11
Catalog：

61262
Application：

WB, FCM
Species Reactivity：

H, M, R

Information

Data

Information

Product Name	KD-Validated F-Box Protein 11 Mouse mAb
Aliases	FBXO11; F-Box Protein 11; FBX11; F-Box Only Protein 11; UBR6; Ubiquitin Protein Ligase E3 Component N-Recognin 6; Protein Arginine N-Methyltransferase 9; Vitiligo-Associated Protein 1; PRMT9; VIT1; Vitiligo-Associated Protein VIT-1; UG063H01; IDDFBA; VIT-1
Background	Gene Name: FBXO11 NCBI Gene Entry: 80204 UniProt Entry: Q86XK2
Application Information	Molecular Weight: Predicted, 104 kDa; observed, 130 kDa Clonality: Mouse monoclonal antibody Clone ID: 25GB520 Species Reactivity: Human,mouse,rat Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	Recombinant protein of human FBXO11
Isotype	Mouse IgG1
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:2,500-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note	This product is for research use only.

Data

Western blotting analysis using FBXO11 antibody (Cat#61262). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FBXO11 antibody (Cat#61262, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using FBXO11 antibody (Cat#61262). FBXO11 expression in wild-type (WT) and FBXO11 knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with FBXO11 antibody (Cat#61262, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of FBXO11 expression in HeLa cells using FBXO11 antibody (Cat# 61262, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, FBXO11.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

FBXO11
Catalog：

61262
Application：

WB, FCM
Species Reactivity：

H, M, R

Datasheet

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