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Home>Product>Primary Antibody

KD-Validated ALDH4A1 Mouse mAb

#61263

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shRNA Knockdown Validated
Western blotting analysis using ALDH4A1 antibody (Cat#61263). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ALDH4A1 antibody (Cat#61263, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using ALDH4A1 antibody (Cat#61263). ALDH4A1 expression in wild-type (WT) and ALDH4A1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ALDH4A1 antibody (Cat#61263, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ALDH4A1
  • Catalog：
    61263
  • Application：
    WB
  • Species Reactivity：
    H, R

Information

Data

Information
Product Name KD-Validated ALDH4A1 Mouse mAb
Aliases ALDH4A1; Aldehyde Dehydrogenase 4 Family Member A1; L-Glutamate Gamma-Semialdehyde Dehydrogenase; P5CDh; ALDH4; Delta-1-Pyrroline-5-Carboxylate Dehydrogenase, Mitochondrial; Aldehyde Dehydrogenase Family 4 Member A1; P5C Dehydrogenase; Mitochondrial Delta-1-Pyrroline 5-Carboxylate Dehydrogenase; Delta-1-Pyrroline-5-Carboxylate Dehydrogenase; Aldehyde Dehydrogenase 4 Family, Member A1; Epididymis Secretory Sperm Binding Protein; EC 1.2.1.88; EC 1.5.1.12; P5CDH; P5CD
Background

Gene Name: ALDH4A1

NCBI Gene Entry: 8659

UniProt Entry: P30038

Application Information

Molecular Weight: Predicted, 62 kDa; observed, 62 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB525

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB)
Immunogen Recombinant protein of human ALDH4A1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:2,500
Note This product is for research use only.
Data
  • Western blotting analysis using ALDH4A1 antibody (Cat#61263). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ALDH4A1 antibody (Cat#61263, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using ALDH4A1 antibody (Cat#61263). ALDH4A1 expression in wild-type (WT) and ALDH4A1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ALDH4A1 antibody (Cat#61263, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ALDH4A1
  • Catalog：
    61263
  • Application：
    WB
  • Species Reactivity：
    H, R
RELATED PRODUCTS
Western blotting analysis using ALDH4A1 antibody (Cat#61263). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ALDH4A1 antibody (Cat#61263, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using ALDH4A1 antibody (Cat#61263). ALDH4A1 expression in wild-type (WT) and ALDH4A1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ALDH4A1 antibody (Cat#61263, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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