KD-Validated PPP3CA Recombinant Rabbit mAb - Product

KD-Validated PPP3CA Recombinant Rabbit mAb

#61275

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Recombinant Antibody
shRNA Knockdown Validated

Western blotting analysis using PPP3CA antibody (Cat#61275). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PPP3CA antibody (Cat#61275, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). PPP3CA, protein phosphatase 3 catalytic subunit alpha.

Western blotting analysis using PPP3CA antibody (Cat#61275). PPP3CA expression in wild type (WT) and PPP3CA shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PPP3CA antibody (Cat#61275, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using PPP3CA antibody (Cat#61275, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Immunocytochemical staining of HeLa cells with PPP3CA antibody (Cat#61275, 1:1,000). Nuclei were stained blue with DAPI; PPP3CA was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.

Flow cytometric analysis of PPP3CA expression in HeLa cells using PPP3CA antibody (Cat#61275, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PPP3CA.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

PPP3CA
Catalog：

61275
Application：

WB, FCM, ICC, IHC-P
Species Reactivity：

H, M

Information

Data

Information

Product Name	KD-Validated PPP3CA Recombinant Rabbit mAb
Aliases	PPP3CA; Protein Phosphatase 3 Catalytic Subunit Alpha; Calcineurin A Alpha; PPP2B; CALNA; CNA1; CAM-PRP Catalytic Subunit; EC 3.1.3.16; CNA Alpha; CALN; Protein Phosphatase 3 (Formerly 2B), Catalytic Subunit, Alpha Isoform (Calcineurin A Alpha); Serine/Threonine-Protein Phosphatase 2B Catalytic Subunit Alpha Isoform; Protein Phosphatase 3 (Formerly 2B), Catalytic Subunit, Alpha Isoform; Protein Phosphatase 2B, Catalytic Subunit, Alpha Isoform; Calmodulin-Dependent Calcineurin A Subunit Alpha Isoform; Protein Phosphatase 3, Catalytic Subunit, Alpha Isozyme; Protein Phosphatase 3 Catalytic Subunit Alpha Isozyme; ACCIID; CALNA1; IECEE1; DEE91; IECEE; CCN1; CNA
Background	Gene Name: PPP3CA NCBI Gene Entry: 5530 UniProt Entry: Q08209
Application Information	Molecular Weight: Predicted, 59 kDa; observed, 59 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2855 Species Reactivity: Human, mouse Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen	A synthesized peptide derived from human Calcineurin A
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000 Immunocytochemistry (ICC): 1:100-1:1,000 Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note	This product is for research use only.

Data

Western blotting analysis using PPP3CA antibody (Cat#61275). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PPP3CA antibody (Cat#61275, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). PPP3CA, protein phosphatase 3 catalytic subunit alpha.
Western blotting analysis using PPP3CA antibody (Cat#61275). PPP3CA expression in wild type (WT) and PPP3CA shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PPP3CA antibody (Cat#61275, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using PPP3CA antibody (Cat#61275, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HeLa cells with PPP3CA antibody (Cat#61275, 1:1,000). Nuclei were stained blue with DAPI; PPP3CA was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of PPP3CA expression in HeLa cells using PPP3CA antibody (Cat#61275, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PPP3CA.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

PPP3CA
Catalog：

61275
Application：

WB, FCM, ICC, IHC-P
Species Reactivity：

H, M

Datasheet

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