Cart

Home

About

Standards

Product

Custom Services

Signal Pathways

Technical Support

News

Sign In/Register

Cart
  • Primary Antibody
  • Secondary Antibodies
  • Lentiviral Particles
  • Stable Cell Lines
  • Lysates
  • Reagents
  • Other Products
Home>Product>Primary Antibody

KD-Validated DDB2 Recombinant Rabbit mAb

#61292

Click to copy product information

Return to List

Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using DDB2 antibody (Cat#61292). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DDB2 antibody (Cat#61292, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). DDB2, damage specific DNA binding protein 2.
Western blotting analysis using DDB2 antibody (Cat#61292). DDB2 expression in wild type (WT) and DDB2 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with DDB2 antibody (Cat#61292, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human gastric carcinoma using DDB2 antibody (Cat#61292, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HT-1080 cells with DDB2 antibody (Cat#61292, 1:1,000). Nuclei were stained blue with DAPI; DDB2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Immunocytochemical staining of HeLa cells using DDB2 antibody (Cat#61292, 1:1,000), Top panel: wild-type (WT); Bottom panal: DDB2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; DDB2 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0. 5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of DDB2 expression in HT-1080 cells using DDB2 antibody (Cat#61292, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, DDB2.
Validation of DDB2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with DDB2 antibody (Cat#61292, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    DDB2
  • Catalog：
    61292
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated DDB2 Recombinant Rabbit mAb
Aliases DDB2; Damage Specific DNA Binding Protein 2; UV-Damaged DNA-Binding Protein 2; DDB P48 Subunit; UV-DDB2; DDBB; XPE; Xeroderma Pigmentosum Group E Protein; DNA Damage-Binding Protein 2; FLJ34321; Damage-Specific DNA Binding Protein 2 (48kD); Damage-Specific DNA Binding Protein 2, 48kDa; Damage-Specific DNA-Binding Protein 2; UV-DDB; DDBb
Background

Gene Name: DDB2

NCBI Gene Entry: 1643

UniProt Entry: Q92466

Application Information

Molecular Weight: Predicted, 48 kDa; observed, 45 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB2805

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human DDB2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using DDB2 antibody (Cat#61292). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DDB2 antibody (Cat#61292, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). DDB2, damage specific DNA binding protein 2.
  • Western blotting analysis using DDB2 antibody (Cat#61292). DDB2 expression in wild type (WT) and DDB2 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with DDB2 antibody (Cat#61292, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human gastric carcinoma using DDB2 antibody (Cat#61292, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunocytochemical staining of HT-1080 cells with DDB2 antibody (Cat#61292, 1:1,000). Nuclei were stained blue with DAPI; DDB2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Immunocytochemical staining of HeLa cells using DDB2 antibody (Cat#61292, 1:1,000), Top panel: wild-type (WT); Bottom panal: DDB2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; DDB2 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0. 5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of DDB2 expression in HT-1080 cells using DDB2 antibody (Cat#61292, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, DDB2.
  • Validation of DDB2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with DDB2 antibody (Cat#61292, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    DDB2
  • Catalog：
    61292
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using DDB2 antibody (Cat#61292). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DDB2 antibody (Cat#61292, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). DDB2, damage specific DNA binding protein 2.
Western blotting analysis using DDB2 antibody (Cat#61292). DDB2 expression in wild type (WT) and DDB2 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with DDB2 antibody (Cat#61292, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human gastric carcinoma using DDB2 antibody (Cat#61292, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HT-1080 cells with DDB2 antibody (Cat#61292, 1:1,000). Nuclei were stained blue with DAPI; DDB2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Immunocytochemical staining of HeLa cells using DDB2 antibody (Cat#61292, 1:1,000), Top panel: wild-type (WT); Bottom panal: DDB2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; DDB2 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0. 5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of DDB2 expression in HT-1080 cells using DDB2 antibody (Cat#61292, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, DDB2.
Validation of DDB2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with DDB2 antibody (Cat#61292, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Shopping cart ()
Estimated total price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$
View cart Empty cart
  • Phone Number

    +1 (301) 545-2395

  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

微信公众号

Copyright © 2025 Hefei GenuIN Biotech Co., Ltd.

All Rights Reserved.