Western blotting analysis using IRAG2 antibody (Cat#5093). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IRAG2 antibody (Cat#5093, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of IRAG2 expression in Raji cells using IRAG2 antibody (Cat#5093, 1:2,000). Green, isotype control; red, IRAG2.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human IRAG2
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:400-1:2,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using IRAG2 antibody (Cat#5093). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IRAG2 antibody (Cat#5093, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of IRAG2 expression in Raji cells using IRAG2 antibody (Cat#5093, 1:2,000). Green, isotype control; red, IRAG2.
Western blotting analysis using IRAG2 antibody (Cat#5093). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IRAG2 antibody (Cat#5093, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of IRAG2 expression in Raji cells using IRAG2 antibody (Cat#5093, 1:2,000). Green, isotype control; red, IRAG2.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.002795s ]
SELECT * FROM `goods` WHERE `code` = '5093' LIMIT 1 [ RunTime:0.003677s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.002383s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000313s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000420s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6256 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000308s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.002123s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6256 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000261s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000404s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.001794s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.078402s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (7750,8797,8473,4916,8412,8730,5765,8184,7680,8588) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000962s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000501s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000244s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000463s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000232s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000315s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000370s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000248s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000208s ]
