Western blotting analysis using SPTAN1 antibody (Cat#64875). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SPTAN1 antibody (Cat#64875, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using SPTAN1 antibody (Cat#64875). SPTAN1 expression in wild-type (WT) and SPTAN1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SPTAN1 antibody (Cat#64875, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using SPTAN1 antibody (Cat#64875, 1:1,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Immunocytochemical staining of C2C12 cells with SPTAN1 antibody (Cat#64875, 1:1,000). Nuclei were stained blue with DAPI; SPTAN1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Immunocytochemical staining of HeLa cells using SPTAN1 antibody (Cat#64875, 1:1,000), Top panel: wild-type (WT); Bottom panal: SPTAN1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; SPTAN1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Validation of SPTAN1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with SPTAN1 antibody (Cat#64875, 1:2,000) and analyzed using BD flow cytometer.
