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  • Primary Antibody
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  • Lentivirus
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  • Lysates
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Home>Product>Primary Antibody

KD-Validated KDM2A Recombinant Rabbit mAb

#65207

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using lysine demethylase 2A antibody (Cat#65207). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with lysine demethylase 2A antibody (Cat#65207, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using lysine demethylase 2A antibody (Cat#65207). lysine demethylase 2A expression in wild-type (WT) and lysine demethylase 2A (KDM2A) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with lysine demethylase 2A antibody (Cat#65207, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with Lysine demethylase 2A antibody (Cat#65207, 1:1,000) . Nuclei were stained blue with DAPI; Lysine demethylase 2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of Lysine demethylase 2A expression in C2C12 cells using Lysine demethylase 2A antibody (Cat#65207, 1:2,000). Green, isotype control; red, Lysine demethylase 2A.
Validation of Lysine demethylase 2A knockdown using flow cytometry. Wild-type(WT, Blue) and Lysine demethylase 2A knockdown(KD, Green) HeLa cells were stained with Lysine demethylase 2A antibody(Cat#65207, 1:2,000) and analyzed using BD flow cytometer.
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    KDM2A
  • Catalog：
    65207
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated KDM2A Recombinant Rabbit mAb
Aliases KDM2A; Lysine Demethylase 2A; JHDM1A; FBL11; CXXC8; FBL7; F-Box And Leucine-Rich Repeat Protein 11; KIAA1004; LILINA; FBXL11; JmjC Domain-Containing Histone Demethylation Protein 1A; Jumonji C Domain-Containing Histone Demethylase 1A; [Histone-H3]-Lysine-36 Demethylase 1A; Lysine (K)-Specific Demethylase 2A; CXXC-Type Zinc Finger Protein 8; Lysine-Specific Demethylase 2A; F-Box/LRR-Repeat Protein 11; DKFZP434M1735; EC 1.14.11.27; FLJ00115; F-Box Protein Lilina; F-Box Protein FBL11; F-Box Protein FBL7
Background

Gene Name: KDM2A

NCBI Gene Entry: 22992

UniProt Entry: Q9Y2K7

Application Information

Molecular Weight: Predicted, 133 kDa, observed, 133 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB160

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human FBXL11
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:100-1:1,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using lysine demethylase 2A antibody (Cat#65207). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with lysine demethylase 2A antibody (Cat#65207, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using lysine demethylase 2A antibody (Cat#65207). lysine demethylase 2A expression in wild-type (WT) and lysine demethylase 2A (KDM2A) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with lysine demethylase 2A antibody (Cat#65207, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of C2C12 cells with Lysine demethylase 2A antibody (Cat#65207, 1:1,000) . Nuclei were stained blue with DAPI; Lysine demethylase 2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Flow cytometric analysis of Lysine demethylase 2A expression in C2C12 cells using Lysine demethylase 2A antibody (Cat#65207, 1:2,000). Green, isotype control; red, Lysine demethylase 2A.
  • Validation of Lysine demethylase 2A knockdown using flow cytometry. Wild-type(WT, Blue) and Lysine demethylase 2A knockdown(KD, Green) HeLa cells were stained with Lysine demethylase 2A antibody(Cat#65207, 1:2,000) and analyzed using BD flow cytometer.
Please select the specifications of the product.
Specification：
20 μL,100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    KDM2A
  • Catalog：
    65207
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using lysine demethylase 2A antibody (Cat#65207). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with lysine demethylase 2A antibody (Cat#65207, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using lysine demethylase 2A antibody (Cat#65207). lysine demethylase 2A expression in wild-type (WT) and lysine demethylase 2A (KDM2A) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with lysine demethylase 2A antibody (Cat#65207, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with Lysine demethylase 2A antibody (Cat#65207, 1:1,000) . Nuclei were stained blue with DAPI; Lysine demethylase 2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of Lysine demethylase 2A expression in C2C12 cells using Lysine demethylase 2A antibody (Cat#65207, 1:2,000). Green, isotype control; red, Lysine demethylase 2A.
Validation of Lysine demethylase 2A knockdown using flow cytometry. Wild-type(WT, Blue) and Lysine demethylase 2A knockdown(KD, Green) HeLa cells were stained with Lysine demethylase 2A antibody(Cat#65207, 1:2,000) and analyzed using BD flow cytometer.
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
View the shopping cart Empty the shopping cart
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    sales@genuinbiotech.com

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    8:30 AM - 5:30 PM EST Mon-Fri

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  132. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-orm/src/db/concern/JoinAndViewQuery.php ( 7.11 KB )
  133. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-orm/src/db/concern/TableFieldInfo.php ( 2.63 KB )
  134. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-orm/src/db/concern/Transaction.php ( 2.77 KB )
  135. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/log/driver/File.php ( 5.96 KB )
  136. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/contract/LogHandlerInterface.php ( 0.86 KB )
  137. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/log/Channel.php ( 3.89 KB )
  138. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/event/LogRecord.php ( 1.02 KB )
  139. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-helper/src/Collection.php ( 16.47 KB )
  140. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-orm/src/db/Raw.php ( 1.22 KB )
  141. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/View.php ( 1.70 KB )
  142. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/View.php ( 4.39 KB )
  143. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/view/driver/Php.php ( 6.44 KB )
  144. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/contract/TemplateHandlerInterface.php ( 1.60 KB )
  145. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public.php ( 3.56 KB )
  146. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public_param.php ( 0.75 KB )
  147. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Request.php ( 9.20 KB )
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  150. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-multi-app/src/Url.php ( 7.52 KB )
  151. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/route/Url.php ( 14.07 KB )
  152. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/head.php ( 3.43 KB )
  153. /www/wwwroot/sbywcs.dsger.com/app/web/view/product/detail.php ( 31.31 KB )
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  1. CONNECT:[ UseTime:0.000492s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000888s ]
  3. SELECT * FROM `goods` WHERE `code` = '65207' LIMIT 1 [ RunTime:0.000497s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000408s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000349s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000407s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 8519 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000449s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000460s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 8519 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000293s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000363s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000273s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.055645s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (35,10505,6535,7264,4035,5566,184,4632,5137,10527) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000836s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000533s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000227s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000523s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000245s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000299s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000379s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000236s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000247s ]
0.094480s