Western blotting analysis using TRAF1 antibody (Cat#5465). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TRAF1 antibody (Cat#5465, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of TRAF1 expression in Raji cells using TRAF1 antibody (Cat#5465, 1:2,000). Green, isotype control; red, TRAF1.
Immunocytochemical staining of Raji cells with TRAF1 antibody (Cat#5465, 1:1,000). Nuclei were stained blue with DAPI; TRAF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using TRAF1 antibody (Cat#5465). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TRAF1 antibody (Cat#5465, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of TRAF1 expression in Raji cells using TRAF1 antibody (Cat#5465, 1:2,000). Green, isotype control; red, TRAF1.
Immunocytochemical staining of Raji cells with TRAF1 antibody (Cat#5465, 1:1,000). Nuclei were stained blue with DAPI; TRAF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using TRAF1 antibody (Cat#5465). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TRAF1 antibody (Cat#5465, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of TRAF1 expression in Raji cells using TRAF1 antibody (Cat#5465, 1:2,000). Green, isotype control; red, TRAF1.
Immunocytochemical staining of Raji cells with TRAF1 antibody (Cat#5465, 1:1,000). Nuclei were stained blue with DAPI; TRAF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
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