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Home>Product>Primary Antibody

KD-Validated CHMP2B Recombinant Rabbit mAb

#61326

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using CHMP2B antibody (Cat#61326). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CHMP2B antibody (Cat#61326, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). CHMP2B, charged multivesicular body protein 2B.
Western blotting analysis using CHMP2B antibody (Cat#61326). CHMP2B expression in wild type (WT) and CHMP2B shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with CHMP2B antibody (Cat#61326, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with CHMP2B antibody (Cat#61326, 1:1,000). Nuclei were stained blue with DAPI; CHMP2B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Immunocytochemical staining of HeLa cells using CHMP2B antibody (Cat#61326, 1:1,000), Top panel: wild-type (WT); Bottom panal: CHMP2B shRNA knockdown (KD). Nuclei were stained blue with DAPI; CHMP2B was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of CHMP2B expression in HepG2 cells using CHMP2B antibody (Cat#61326, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CHMP2B.
Validation of CHMP2B knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with CHMP2B antibody (Cat#61326, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
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  • Gene Symbol：
    CHMP2B
  • Catalog：
    61326
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated CHMP2B Recombinant Rabbit mAb
Aliases CHMP2B; Charged Multivesicular Body Protein 2B; CHMP2.5; VPS2B; Charged Multivesicular Body Protein 2b; Chromatin Modifying Protein 2B; DKFZP564O123; Vacuolar Protein-Sorting-Associated Protein 2-2; Vacuolar Protein Sorting-Associated Protein 2-2; VPS2 Homolog B (S. Cerevisiae); Chromatin-Modifying Protein 2b; VPS2 Homolog B; FTDALS7; HVps2-2; VPS2-2; CHMP2b; Vps2-2; ALS17; DMT1
Background

Gene Name: CHMP2B

NCBI Gene Entry: 25978

UniProt Entry: Q9UQN3

Application Information

Molecular Weight: Predicted, 24 kDa; observed, 28 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB3070

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from CHMP2B
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using CHMP2B antibody (Cat#61326). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CHMP2B antibody (Cat#61326, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). CHMP2B, charged multivesicular body protein 2B.
  • Western blotting analysis using CHMP2B antibody (Cat#61326). CHMP2B expression in wild type (WT) and CHMP2B shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with CHMP2B antibody (Cat#61326, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with CHMP2B antibody (Cat#61326, 1:1,000). Nuclei were stained blue with DAPI; CHMP2B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Immunocytochemical staining of HeLa cells using CHMP2B antibody (Cat#61326, 1:1,000), Top panel: wild-type (WT); Bottom panal: CHMP2B shRNA knockdown (KD). Nuclei were stained blue with DAPI; CHMP2B was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Flow cytometric analysis of CHMP2B expression in HepG2 cells using CHMP2B antibody (Cat#61326, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CHMP2B.
  • Validation of CHMP2B knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with CHMP2B antibody (Cat#61326, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CHMP2B
  • Catalog：
    61326
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using CHMP2B antibody (Cat#61326). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CHMP2B antibody (Cat#61326, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). CHMP2B, charged multivesicular body protein 2B.
Western blotting analysis using CHMP2B antibody (Cat#61326). CHMP2B expression in wild type (WT) and CHMP2B shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with CHMP2B antibody (Cat#61326, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with CHMP2B antibody (Cat#61326, 1:1,000). Nuclei were stained blue with DAPI; CHMP2B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Immunocytochemical staining of HeLa cells using CHMP2B antibody (Cat#61326, 1:1,000), Top panel: wild-type (WT); Bottom panal: CHMP2B shRNA knockdown (KD). Nuclei were stained blue with DAPI; CHMP2B was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of CHMP2B expression in HepG2 cells using CHMP2B antibody (Cat#61326, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CHMP2B.
Validation of CHMP2B knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with CHMP2B antibody (Cat#61326, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
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