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Home>Product>Primary Antibody

KD-Validated IKK α Recombinant Rabbit mAb

#61374

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using IKK α antibody (Cat#61374). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IKK α antibody (Cat#61374,1:20,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201,1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using IKK alpha antibody (Cat#61374). IKK alpha expression in wild type (WT) and IKK alpha shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with IKK alpha antibody (Cat#61374,1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201,1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with IKK alpha antibody (Cat#61374, 1:1,000). Nuclei were stained blue with DAPI; IKK alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Immunocytochemical staining of HeLa cells using IKK alpha antibody (Cat#61374, 1:1,000), Top panel: wild-type (WT); Bottom panal: IKK alpha shRNA knockdown (KD). Nuclei were stained blue with DAPI; IKKalpha was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of IKK alpha expression in HepG2 cells using IKK alpha antibody (Cat#61374, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, IKK alpha.
Validation of IKK alpha knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with IKK alpha antibody (Cat#61374, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CHUK
  • Catalog：
    61374
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated IKK α Recombinant Rabbit mAb
Aliases CHUK; Component Of Inhibitor Of Nuclear Factor Kappa B Kinase Complex; IKK-Alpha; NFKBIKA; IKK1; IKKA; Inhibitor Of Nuclear Factor Kappa-B Kinase Subunit Alpha; Conserved Helix-Loop-Helix Ubiquitous Kinase; IkBKA; TCF16; Transcription Factor 16; I-Kappa-B Kinase 1; EC 2.7.11.10; IKK-1; Nuclear Factor NF-Kappa-B Inhibitor Kinase Alpha; Nuclear Factor NFkappaB Inhibitor Kinase Alpha; IkB Kinase Alpha Subunit; I-Kappa-B Kinase-Alpha; I-Kappa-B Kinase Alpha; I-Kappa-B Kinase; IkappaB Kinase; IKK-A Kinase; EC 2.7.11; TCF-16; IKBKA; IKK-A; BPS2
Background

Gene Name: CHUK

NCBI Gene Entry: 1147

UniProt Entry: O15111

Application Information

Molecular Weight: Predicted, 85 kDa; observed, 85 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB765

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human IKK α
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:4,000-1:20,000
Flow Cytometry (FCM): 1:100-1:1,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using IKK α antibody (Cat#61374). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IKK α antibody (Cat#61374,1:20,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201,1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using IKK alpha antibody (Cat#61374). IKK alpha expression in wild type (WT) and IKK alpha shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with IKK alpha antibody (Cat#61374,1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201,1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with IKK alpha antibody (Cat#61374, 1:1,000). Nuclei were stained blue with DAPI; IKK alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Immunocytochemical staining of HeLa cells using IKK alpha antibody (Cat#61374, 1:1,000), Top panel: wild-type (WT); Bottom panal: IKK alpha shRNA knockdown (KD). Nuclei were stained blue with DAPI; IKKalpha was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Flow cytometric analysis of IKK alpha expression in HepG2 cells using IKK alpha antibody (Cat#61374, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, IKK alpha.
  • Validation of IKK alpha knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with IKK alpha antibody (Cat#61374, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CHUK
  • Catalog：
    61374
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using IKK α antibody (Cat#61374). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IKK α antibody (Cat#61374,1:20,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201,1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using IKK alpha antibody (Cat#61374). IKK alpha expression in wild type (WT) and IKK alpha shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with IKK alpha antibody (Cat#61374,1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201,1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with IKK alpha antibody (Cat#61374, 1:1,000). Nuclei were stained blue with DAPI; IKK alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Immunocytochemical staining of HeLa cells using IKK alpha antibody (Cat#61374, 1:1,000), Top panel: wild-type (WT); Bottom panal: IKK alpha shRNA knockdown (KD). Nuclei were stained blue with DAPI; IKKalpha was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of IKK alpha expression in HepG2 cells using IKK alpha antibody (Cat#61374, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, IKK alpha.
Validation of IKK alpha knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with IKK alpha antibody (Cat#61374, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
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