Western blotting analysis using Integrin β3 antibody (Cat#68733). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Integrin β3 antibody (Cat#68733, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using integrin beta 3 antibody (Cat#68733). Integrin beta 3 expression in wild-type (WT) and integrin beta 3 (ITGB3) shRNA knockdown (KD) C2C12 cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with integrin beta 3 antibody (Cat#68733, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with Integrin beta 3 antibody (Cat#68733, 1:1,000). Nuclei were stained blue with DAPI; Integrin beta 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of Integrin beta 3 expression in C2C12 cells using Integrin beta 3 antibody (Cat#68733, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Integrin beta 3.
