Western blotting analysis using myelin associated glycoprotein antibody (Cat#61466). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with myelin associated glycoprotein antibody (Cat#61466, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using myelin associated glycoprotein antibody (Cat#61466). Myelin associated glycoprotein expression in wild type (WT) and myelin associated glycoprotein (MAG) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with myelin associated glycoprotein antibody (Cat#61466, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Myelin associated glycoprotein expression in HeLa cells using Myelin associated glycoprotein antibody (Cat#61466, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Myelin associated glycoprotein.
