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Home>Product>Primary Antibody

KD-Validated Grp75 Recombinant Rabbit mAb

#61525

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using Grp75 antibody (Cat#61525). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Grp75 antibody (Cat#61525, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Grp75 antibody (Cat#61525). Grp75 expression in wild type (WT) and Grp75 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Grp75 antibody (Cat#61525, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Grp75 antibody (Cat#61525, 1:1,000). Nuclei were stained blue with DAPI; Grp75 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HSPA9
  • Catalog：
    61525
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M

Information

Data

Information
Product Name KD-Validated Grp75 Recombinant Rabbit mAb
Aliases HSPA9; Heat Shock Protein Family A (Hsp70) Member 9; GRP75; PBP74; 75 KDa Glucose-Regulated Protein; Stress-70 Protein, Mitochondrial; HSPA9B; Heat Shock 70kDa Protein 9 (Mortalin); Peptide-Binding Protein 74; Mortalin2; Mortalin; MTHSP75; GRP-75; MOT; Epididymis Secretory Sperm Binding Protein Li 124m; Heat Shock 70kDa Protein 9B (Mortalin-2); Catecholamine-Regulated Protein 40; Heat Shock 70 KDa Protein 9; Heat Shock 70kD Protein 9B; Mortalin, Perinuclear; P66-Mortalin; HEL-S-124m; Mortalin-2; Mt-HSP70; Mthsp75; SIDBA4; Mot-2; CRP40; EVPLS; MOT-2; MOT2; SAAN; CSA
Background

Gene Name: HSPA9

NCBI Gene Entry: 3313

UniProt Entry: P38646

Application Information

Molecular Weight: Predicted, 74 kDa; observed, 74 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB2615

Species Reactivity: Human,mouse

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Grp75
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Grp75 antibody (Cat#61525). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Grp75 antibody (Cat#61525, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using Grp75 antibody (Cat#61525). Grp75 expression in wild type (WT) and Grp75 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Grp75 antibody (Cat#61525, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with Grp75 antibody (Cat#61525, 1:1,000). Nuclei were stained blue with DAPI; Grp75 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HSPA9
  • Catalog：
    61525
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M
RELATED PRODUCTS
Western blotting analysis using Grp75 antibody (Cat#61525). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Grp75 antibody (Cat#61525, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Grp75 antibody (Cat#61525). Grp75 expression in wild type (WT) and Grp75 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Grp75 antibody (Cat#61525, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Grp75 antibody (Cat#61525, 1:1,000). Nuclei were stained blue with DAPI; Grp75 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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