Western blotting analysis using fibroblast growth factor receptor 3 antibody (Cat#61593). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with fibroblast growth factor receptor 3 antibody (Cat#61593, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using fibroblast growth factor receptor 3 antibody (Cat#61593). Fibroblast growth factor receptor 3 expression in wild-type (WT) and fibroblast growth factor receptor 3 (FGFR3) knockdown (KD) 293T cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with fibroblast growth factor receptor 3 antibody (Cat#61593, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with Fibroblast growth factor receptor 3 antibody (Cat#61593, 1:1,000). Nuclei were stained blue with DAPI; Fibroblast growth factor receptor 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of Fibroblast growth factor receptor 3 expression in C2C12 cells using Fibroblast growth factor receptor 3 antibody (Cat#61593, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Fibroblast growth factor receptor 3.
