Western blotting analysis using Clathrin heavy chain antibody (Cat#61598). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Clathrin heavy chain antibody (Cat#61598, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Clathrin heavy chain antibody (Cat#61598). Clathrin heavy chain expression in wild type (WT) and Clathrin heavy chain shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Clathrin heavy chain antibody (Cat#61598, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Clathrin heavy chain antibody (Cat#61598, 1:1000). Nuclei were stained blue with DAPI; Clathrin heavy chain was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of Clathrin heavy chain expression in C2C12 cells using Clathrin heavy chain antibody (Cat#61598, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Clathrin heavy chain.
